Partial purification of alkaline protease by mutant strain of Bacillus subtilis
نویسنده
چکیده
The present study utilized different methods for purification of alkaline protease by using ammonium sulphate and acetone. Ammonium sulphate was found the best purifying agent and gave 109.09 fold purification of protease with 49.7% yield at 60% saturation. Dialysis was performed by using phosphate and Tris-HCl buffer and phosphate buffer was optimized to good dialyzing agent at pH 8.0.
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